Molecular cloning of cDNA encoding the c-kit receptor of Shiba goats and a novel alanine insertion specific to goats and sheep in the kinase insert region

by

The complete open reading frame (ORF) of the c-kit cDNA was cloned from a cerebellar cDNA library of the Shiba goat (Capra hircus var Shiba) with the dominant black-eyed white phenotype. The analysis of the deduced amino acid sequence revealed the presence of a single amino acid insertion (alanine) in the kinase insert (KI) region. … Read more

cDNA synthesis for BCR-ABL1 detection at the MMR level: the importance of using the appropriate kit

by

BACKGROUND The synthesis of complementary DNA (cDNA) for use in the detection of BCR-ABL1 at the Major Molecular Response (MMR) level is a well-established method used by clinical laboratories world-wide. However, the quality of cDNA provides sensitivity challenges and consequently affects the detection of Minimal Residual Disease (MRD). RESULTS Herein, we evaluated six commercially available … Read more

Novel polygenic risk score approach with transcriptome-based weighting for genetic risk prediction of late-onset Alzheimer’s disease

by

Background: Recent genetic studies showed that polygenic risk score (PRS) could be used to identify individuals at high risk of Alzheimer’s disease (AD). Despite this success, early detection and intervention of AD still remain challenging. In this study, we suggest a novel PRS approach that is weighted by predicted tissue-specific gene expression levels. Method: We used … Read more

SARS-CoV-2 full-length Trimeric Spike Recombinant Antigen B.1.617.2 Mutation (Delta Variant)

by

Peak protein from the mutant strain B.1.617.2, also commonly known as the “Delta Variant”. It is a full-length protein, which is active in its native trimeric form, which is stabilized in LMNG detergent. All viruses undergo rapid mutations and adapt quickly to the countermeasures that the immune system creates against them. The SARS-CoV-2 of the … Read more

CYP2C9 QC

by

CYP2C9 is a phase I drug-metabolizing cytochrome P450 (CYP450) enzyme isoform that plays a major role in the oxidation of both xenobiotic and endogenous compounds. Gray et al. identified CYP2C9 as one of several CYP2C genes clustered in a 500 kb region on chromosome 10q24. The cluster comprises four genes arranged in the order CYP2C8-CYP2C9-CYP2C19-CYP2C18. Several studies identified a single … Read more

Semantic Indexing of Medical Learning Objects: Medical Students’ Usage of a Semantic Network.

by
Semantic Indexing of Medical Learning Objects: Medical Students' Usage of a Semantic Network.

BACKGROUNDThe Semantically Annotated Media (SAM) undertaking goals to supply a versatile platform for looking, searching, and indexing medical studying objects (MLOs) based mostly on a semantic community derived from established classification programs. Primarily, SAM helps the Aachen emedia expertise lab, however SAM is prepared for indexing distributed content material and the Simple Knowledge Organizing System … Read more

Using citizen science to expand the global map of landslides: Introducing the Cooperative Open Online Landslide Repository (COOLR).

by
Using citizen science to expand the global map of landslides: Introducing the Cooperative Open Online Landslide Repository (COOLR).

Robust inventories are very important for enhancing evaluation of and response to lethal and dear landslide hazards. However, accumulating landslide occasions in inventories is troublesome at the global scale due to inconsistencies in or the absence of landslide reporting. Citizen science is a beneficial alternative for addressing some of these challenges. The new Cooperative Open Online Landslide Repository (COOLR) … Read more

Geographic review on the specimens of the Caatinga Biome in the Jardim Botânico do Rio de Janeiro (RB) herbarium.

by
Geographic review on the specimens of the Caatinga Biome in the Jardim Botânico do Rio de Janeiro (RB) herbarium.

This article provides a quantitative description of flora specimens saved in the Jardim Botânico of Rio de Janeiro Herbarium that belongs to the Federal Conservation Units of Caatinga’s phytogeography space. The Caatinga represents 11% of Brazilian territory and is, in South America, the largest and most biodiverse semi-arid tropical ecoregion, however solely 5% of its … Read more