TGF-β1-induced Collagen Promotes Chicken Ovarian Follicle Development via an Intercellular Cooperative Pattern

by

Follicle development is a complex process under strict regulation of diverse hormones and cytokines including transforming growth factor β (TGF-β) superfamily members. TGF-β is pivotal for the regulation of ovarian functions under physiological and pathological conditions. In this study, effect of TGF-β1 on chicken follicle development was examined through investigating the accumulation and action of … Read more

Different protective efficacies of a novel antigen-specific DNA vaccine encoding chicken type Ⅱ collagen via intramuscular, subcutaneous, and intravenous vaccination against experimental rheumatoid arthritis

by

Tolerizing DNA vaccines encoding key autoantigens are one of emerging strategies for the treatment of rheumatoid arthritis (RA). Among these vaccines, the most representative is pcDNA-CCOL2A1, an antigen-specific DNA vaccine encoding chicken type Ⅱ collagen (CCⅡ) with significant therapeutic and prophylactic efficacy in collagen-induced arthritis (CIA) rat models. We compared the in situ expression levels … Read more

Immunization of mice with the self-peptide ACBP coupled to keyhole limpet hemocyanin

by

Keyhole limpet hemocyanin (KLH) is a glycosylated multi-subunit metalloprotein that elicits a strong nonspecific immune activation, thus inducing both cellular and humoral immune responses. The exceptional immunogenicity of this protein can be leveraged to vaccinate mice against self-antigens that otherwise would not induce an autoimmune response. This protocol describes the covalent conjugation of KLH with … Read more

Novel disintegrin-like peptides derived from an amphibian skin cDNA sequence of Hypsiboas punctatus

by

Disintegrins comprise a family of small proteins that bind to and alter the physiological function of integrins, especially integrins that mediate platelet aggregation in blood. Here, we report a lysine-glycine-aspartic acid (KGD) disintegrin-like motif present in a 15-amino acid residue peptide identified in a cDNA library of the amphibian Hypsiboas punctatus skin. The original peptide … Read more

Molecular cloning of cDNA encoding the c-kit receptor of Shiba goats and a novel alanine insertion specific to goats and sheep in the kinase insert region

by

The complete open reading frame (ORF) of the c-kit cDNA was cloned from a cerebellar cDNA library of the Shiba goat (Capra hircus var Shiba) with the dominant black-eyed white phenotype. The analysis of the deduced amino acid sequence revealed the presence of a single amino acid insertion (alanine) in the kinase insert (KI) region. … Read more

cDNA synthesis for BCR-ABL1 detection at the MMR level: the importance of using the appropriate kit

by

BACKGROUND The synthesis of complementary DNA (cDNA) for use in the detection of BCR-ABL1 at the Major Molecular Response (MMR) level is a well-established method used by clinical laboratories world-wide. However, the quality of cDNA provides sensitivity challenges and consequently affects the detection of Minimal Residual Disease (MRD). RESULTS Herein, we evaluated six commercially available … Read more

Novel polygenic risk score approach with transcriptome-based weighting for genetic risk prediction of late-onset Alzheimer’s disease

by

Background: Recent genetic studies showed that polygenic risk score (PRS) could be used to identify individuals at high risk of Alzheimer’s disease (AD). Despite this success, early detection and intervention of AD still remain challenging. In this study, we suggest a novel PRS approach that is weighted by predicted tissue-specific gene expression levels. Method: We used … Read more

SARS-CoV-2 full-length Trimeric Spike Recombinant Antigen B.1.617.2 Mutation (Delta Variant)

by

Peak protein from the mutant strain B.1.617.2, also commonly known as the “Delta Variant”. It is a full-length protein, which is active in its native trimeric form, which is stabilized in LMNG detergent. All viruses undergo rapid mutations and adapt quickly to the countermeasures that the immune system creates against them. The SARS-CoV-2 of the … Read more

CYP2C9 QC

by

CYP2C9 is a phase I drug-metabolizing cytochrome P450 (CYP450) enzyme isoform that plays a major role in the oxidation of both xenobiotic and endogenous compounds. Gray et al. identified CYP2C9 as one of several CYP2C genes clustered in a 500 kb region on chromosome 10q24. The cluster comprises four genes arranged in the order CYP2C8-CYP2C9-CYP2C19-CYP2C18. Several studies identified a single … Read more

Semantic Indexing of Medical Learning Objects: Medical Students’ Usage of a Semantic Network.

by
Semantic Indexing of Medical Learning Objects: Medical Students' Usage of a Semantic Network.

BACKGROUNDThe Semantically Annotated Media (SAM) undertaking goals to supply a versatile platform for looking, searching, and indexing medical studying objects (MLOs) based mostly on a semantic community derived from established classification programs. Primarily, SAM helps the Aachen emedia expertise lab, however SAM is prepared for indexing distributed content material and the Simple Knowledge Organizing System … Read more