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   COLLABORATIVE PROJECTS

Interbio aims at promoting interdisciplinary and interregional research in the field of health & life sciences. Interbio can provide mobility support (transport & accommodation) for scientists interested in setting up collaborative research projects.
For more information, please contact your local WP3 coordinator.

GT4.- Proteomic analysis of cellular responses to protein-aggregation-mediated stress

Partner A Member : Barcelona
Name of principal investigator : VENTURA ZAMORA
FirstName : Salvador
E-mail : Salvador.Ventura@uab.es
Position : Staff
Affiliation Name : UAB
Road : Cerdanyola del Vallès
Postbox :
Town : Bellaterra
Country : Spain
Partner B Member : Lisboa
Name of principal investigator : VARELA COELHO
FirstName : Ana
E-mail : varela@itqb.unl.pt
Position : Staff
Affiliation Name : Instituto Tecnologia Química e Biológica
Road : Av República, EAN
Postbox :
Town : Oeiras
Country : Lisbon
Request for financial support 1 scientist for 90 days (Partner A to Partner B)
Perspectives of extension of this common project to a more ambitious proposal to be submitted to funding agencies : Long term
Added value of the cooperation : exchange of expertise

Summary of the proposal : Protein aggregation is related with an increasing number of human diseases.Our purpose is to determine if there is a common response against protein aggregation and to identify the proteins involved.

To achieve this we wanted to compare the proteomic response related with the aggregation of Transthyretin (associated with senile systemic amyloidosis) and amyloid-β-peptide (Alzheimer’s disease) in yeast. The changes at the proteome level will be analyzed by comparing the protein distribution in cells expressing aggregating and non aggregating versions of the protein. Cells would be grown in controlled chemostats and high-throughput differential proteomics analysis will be applied; specifically the 2D-DIGE (Differential in Gel Electrophoresis) methodology will be used. The 2D-DIGE technique requires the use of a scanner with multi-wavelength fluorescence detection, available at ITQB. After the 2D electrophoretic separation, gels will be scanned with a high resolution fluorescent scanner (Fuji Film Fla-5100) at different wavelengths depending on the dye used. Subsequently, images will be analyzed with 2DE software Progenesis Samespots (Nonlinear Dynamics) in order to assess significant differences in protein expression (spot intensities).

Quantitative differences will be evaluated by ANOVA and t-student tests. Cluster and principal component analysis will also be used to verify data quality. The protein spots shown to be differential expressed will be identified using a combined analysis of PMF (Peptide Mass Fingerprint) and tandem mass data, acquired by a MALDI/TOF-TOF mass spectrometer (model 4800, Applied Biosystems) and by a linear ion trap mass spectrometer with electrospray ionization (model LTQ, Thermo-Finnigan) coupled to a micro-RP HPLC (Thermo-Finnigan). Several software for protein identification, ProteinPilot using the Paragon™ algorithm (Applied Biosystems); on-line MASCOT; GPS (Applied Biosystems) and Bioworks™ 3.3.1 (Thermo-Finnigan) will be used in order to achieve better confidence in protein identification.

View document
 
Antonio PINEDA-LUCENA, Valencia
Claudio GOMES, Lisboa
Using metabonomics to understand fatty acid oxidation disease View proposal
Miriam ROYO EXPOSITO, Barcelona
Ivan HUC, Bordeaux
Exploring delta-aromatic carboxamide foldamers and alpha-peptides compatibility View proposal
Salvador VENTURA ZAMORA, Barcelona
Ana VARELA COELHO, Lisboa
GT4.- Proteomic analysis of cellular responses to protein-aggregation-mediated stress View proposal
Olivier GADAL, Toulouse
Marc MARTI-RENOM, Valencia
Integrating probabilistic maps and 3C-based data for determining high-resolution 3D structure of yeast chromosomes View proposal
Yann ASTIER, Lisboa
Josep SAMITIER, Barcelona
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Jorge PINTO PAIVA, Lisboa
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Royo BEATRIZ , Lisboa
Gómez MONTSERRAT, Toulouse
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Elio SUCENA, Lisboa
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Gilles GUICHARD, Bordeaux
Fernando ALBERICIO, Barcelona
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Bruno BRUNO VICTOR, Lisboa
Xavier XAVIER DAURA, Barcelona
Molecular Simulations of Hemagglutinin from Influenza virus View proposal
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Lucas DEL CASTILLO AGUDO, Valencia
WG1 Comparative proteome analysis of C. albicans strains SC5314 and CAI4 View proposal
Peter FALLER, Toulouse
Claudio GOMES, Lisboa
Structural and functional aspects of the Cu/Fe-amyloid-beta complex View proposal
Egea GUSTAVO, Barcelona
Elisabeth GENOT, Bordeaux
TGFbeta signaling in cardiovascular alterations associated with the Marfan syndrome View proposal
Cecilia ARRAIANO, Lisboa
Muriel COCAIGN-BOUSQUET, Toulouse
Stabilome E coli View proposal
Muñoz-almagro CARMEN , Barcelona
Sa-leao RAQUEL, Lisboa
Invasiveness of pneumococcal serotypes and clones circulating in Catalonia during heptavalente conjugate vaccine period View proposal
Olga IRANZO, Lisboa
Rafel PROHENS, Barcelona
Study of the influence of peptide flexibility on metal ion coordination properties View proposal
Rita ABRANCHES, Lisboa
José maría SEGUÍ, Valencia
Investigating the subcellular trafficking of human recombinant proteins expressed in plants View proposal
Francesc PALAU, Valencia
Cláudio GOMES, Lisboa
Evaluation of mitochondrial beta-oxidation flavoenzymes in a cellular model of Friedreich ataxia View proposal
Jean-jacques TOULMÉ, Bordeaux
Claudio GOMES, Lisboa
Studies on human S100 protein-aptamer complexes View proposal
Justine BERTRAND-MICHEL, Toulouse
Gemma FABRIÀS, Barcelona
WP4 Sphingolipidomic technical exchange View proposal
Célia ROMÃO, Lisboa
Jordi juanhuix GIBERT, Barcelona
Introduction to the MX XALOC BL13 Beamline at ALBA View proposal
   

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