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Interbio aims at promoting interdisciplinary and interregional research in the field of health & life sciences. Interbio can provide mobility support (transport & accommodation) for scientists interested in setting up collaborative research projects.
For more information, please contact your local WP3 coordinator.

GT4.- Proteomic analysis of cellular responses to protein-aggregation-mediated stress

Partner A Member : Barcelona
Name of principal investigator : VENTURA ZAMORA
FirstName : Salvador
E-mail : Salvador.Ventura@uab.es
Position : Staff
Affiliation Name : UAB
Road : Cerdanyola del Vallès
Postbox :
Town : Bellaterra
Country : Spain
Partner B Member : Lisboa
Name of principal investigator : VARELA COELHO
FirstName : Ana
E-mail : varela@itqb.unl.pt
Position : Staff
Affiliation Name : Instituto Tecnologia Química e Biológica
Road : Av República, EAN
Postbox :
Town : Oeiras
Country : Lisbon
Request for financial support 1 scientist for 90 days (Partner A to Partner B)
Perspectives of extension of this common project to a more ambitious proposal to be submitted to funding agencies : Long term
Added value of the cooperation : exchange of expertise

Summary of the proposal : Protein aggregation is related with an increasing number of human diseases.Our purpose is to determine if there is a common response against protein aggregation and to identify the proteins involved.

To achieve this we wanted to compare the proteomic response related with the aggregation of Transthyretin (associated with senile systemic amyloidosis) and amyloid-β-peptide (Alzheimer’s disease) in yeast. The changes at the proteome level will be analyzed by comparing the protein distribution in cells expressing aggregating and non aggregating versions of the protein. Cells would be grown in controlled chemostats and high-throughput differential proteomics analysis will be applied; specifically the 2D-DIGE (Differential in Gel Electrophoresis) methodology will be used. The 2D-DIGE technique requires the use of a scanner with multi-wavelength fluorescence detection, available at ITQB. After the 2D electrophoretic separation, gels will be scanned with a high resolution fluorescent scanner (Fuji Film Fla-5100) at different wavelengths depending on the dye used. Subsequently, images will be analyzed with 2DE software Progenesis Samespots (Nonlinear Dynamics) in order to assess significant differences in protein expression (spot intensities).

Quantitative differences will be evaluated by ANOVA and t-student tests. Cluster and principal component analysis will also be used to verify data quality. The protein spots shown to be differential expressed will be identified using a combined analysis of PMF (Peptide Mass Fingerprint) and tandem mass data, acquired by a MALDI/TOF-TOF mass spectrometer (model 4800, Applied Biosystems) and by a linear ion trap mass spectrometer with electrospray ionization (model LTQ, Thermo-Finnigan) coupled to a micro-RP HPLC (Thermo-Finnigan). Several software for protein identification, ProteinPilot using the Paragon™ algorithm (Applied Biosystems); on-line MASCOT; GPS (Applied Biosystems) and Bioworks™ 3.3.1 (Thermo-Finnigan) will be used in order to achieve better confidence in protein identification.

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Antonio PINEDA-LUCENA, Valencia
Claudio GOMES, Lisboa
Using metabonomics to understand fatty acid oxidation disease View proposal
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Ivan HUC, Bordeaux
Exploring delta-aromatic carboxamide foldamers and alpha-peptides compatibility View proposal
Salvador VENTURA ZAMORA, Barcelona
GT4.- Proteomic analysis of cellular responses to protein-aggregation-mediated stress View proposal
Olivier GADAL, Toulouse
Marc MARTI-RENOM, Valencia
Integrating probabilistic maps and 3C-based data for determining high-resolution 3D structure of yeast chromosomes View proposal
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Josep SAMITIER, Barcelona
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Jorge PINTO PAIVA, Lisboa
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Gómez MONTSERRAT, Toulouse
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Elio SUCENA, Lisboa
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Claudio GOMES, Lisboa
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Egea GUSTAVO, Barcelona
Elisabeth GENOT, Bordeaux
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Cecilia ARRAIANO, Lisboa
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Muñoz-almagro CARMEN , Barcelona
Sa-leao RAQUEL, Lisboa
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Rafel PROHENS, Barcelona
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Rita ABRANCHES, Lisboa
José maría SEGUÍ, Valencia
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Francesc PALAU, Valencia
Cláudio GOMES, Lisboa
Evaluation of mitochondrial beta-oxidation flavoenzymes in a cellular model of Friedreich ataxia View proposal
Jean-jacques TOULMÉ, Bordeaux
Claudio GOMES, Lisboa
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Justine BERTRAND-MICHEL, Toulouse
Gemma FABRIÀS, Barcelona
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Jordi juanhuix GIBERT, Barcelona
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