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Studies on human S100 protein-aptamer complexes
Perspectives of extension of this common project to a more ambitious proposal to be submitted to funding agencies : Short term Added value of the cooperation : ITQB has the proteins, IECB the aptamer.
Summary of the proposal :
The S100 proteins are a multi-gene calcium-binding family comprising 20 known human members each coded by a separate gene. They are small, acidic proteins found exclusively in vertebrates. The expression of S100 proteins has been demonstrated in a diverse spectrum of tissues. S100 proteins have a broad range of intracellular and extracellular functions, including include regulation of protein phosphorylation and enzyme activity, calcium homeostasis, regulation of cytoskeletal components and regulation of transcriptional factors. Some members of the family act as leukocyte chemoattractants, macrophage activators and modulators of cell proliferation. S100 proteins are associated with a variety of pathologies such as inflammation and cardiomyopathies, and gradually their role in carcinogenesis is unraveled.
In the frame of a previous project aiming at the identification of aptamers to membrane proteins the IECB team raised aptamers that proteomics analysis tentatively identified as a S100 ligands. The binding properties and the specificity of these aptamers were not determined. It turns out that the ITQB team develops investigations on this class of proteins. This collaborative project aims firstly at characterizing the S100-aptamer complexes. Surface Plasmon Resonance analysis will be carried out on the IECB platform in order to evaluate the equilibrium constant of the complexes. Various S100 proteins will be assayed. Once characterized these aptamers could be used by the ITQB team for structure/function studies and by the IECB team for biotechnological developments (targeting, imaging).
Antonio PINEDA-LUCENA, Valencia Claudio GOMES, Lisboa
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