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   COLLABORATIVE PROJECTS

Interbio aims at promoting interdisciplinary and interregional research in the field of health & life sciences. Interbio can provide mobility support (transport & accommodation) for scientists interested in setting up collaborative research projects.
For more information, please contact your local WP3 coordinator.

Invasiveness of pneumococcal serotypes and clones circulating in Catalonia during heptavalente conjugate vaccine period

Partner A Member : Barcelona
Name of principal investigator : CARMEN
FirstName : Muñoz-almagro
E-mail : cma@hsjdbcn.org
Position : Staff
Affiliation Name : Hospital Sant Joan de Deu
Road : Pº Sant Joan de Deu Nº2
Postbox :
Town : Esplugues de Llobregat, Barcelona
Country : Spain
Partner B Member : Lisboa
Name of principal investigator : RAQUEL
FirstName : Sa-leao
E-mail : rsaleao@itqb.unl.pt
Position : Staff
Affiliation Name : Laboratory of Molecular Microbiology – ITQB
Road : Rua da Quinta Grande, 6
Postbox :
Town : Oeiras
Country : Lisbon
Request for financial support 1 scientist for 15 days (Partner A to Partner B)
Perspectives of extension of this common project to a more ambitious proposal to be submitted to funding agencies : Long term
Added value of the cooperation : The objective of this collaboration is to exchange information and know-how through hand-on experiments about: 1) Real-Time PCR applications for pneumococcal detection and molecular serotyping 2) Serotyping methods based on bacterial culture 3) Detection and characterization of atypical pneumococci 4) Application of Simpson’s diversity index and other coefficients to compare pneumococcal collections

Summary of the proposal : Dr. Raquel Sá-Leão from the Laboratory of Molecular Microbiology of ITQB and Prof. Hermínia de Lencastre head of the Molecular Microbiology of Human Pathogens Unit are worldwide recognized authorities in the field of pneumococcal infections. They have important experience about genetic studies related to the diversity of pneumococcal populations.

PIDRG-HSJD is an emergent research group in the field of pneumococci in Spain. This group has a well characterized collection of invasive and carriage pneumococcal isolates among children from Catalonia, during the period 2004-2009. In addition, the molecular microbiology department of HSJD has wide experience in the use of molecular microbiology techniques such as Real-Time PCR and sequence-based methods  for the diagnosis and characterization of main pediatric infections. In the field of pneumococci, Real-Time PCR is applied for diagnosis of pneumococci in direct biological samples (plasma, cerebrospinal fluid or pleural fluid among others) molecular capsular type detection of pneumococci and pneumococcal bacterial load quantification.  Automatic sequencing is applied for clonal type detection of pneumococci by MLST.

The objective of this collaboration is to exchange information and know-how through hand-on experiments about:

1) Real-Time PCR applications for pneumococcal detection and molecular serotyping

2) Serotyping methods based on bacterial culture

3) Detection and characterization of atypical pneumococci

4) Application of Simpson’s diversity index and other coefficients to compare pneumococcal collections

To develop this collaboration, Dra. Carmen Muñoz-Almagro, Head of PIDRG-HSJD and responsible of the Molecular Microbiology Department of HSJD, will make a formative internship next July 2011 in ITQB in Oeiras, Portugal.

This stage will serve to complete the study “ Invasiveness of pneumococcal serotypes and clones circulating in Catalonia during heptavalent conjugate vaccine period” performed by Carmen Muñoz-Almagro and the PIDRG-HSJD under the supervision and collaboration of Dr Raquel Sá-Leão and Prof. Hermínia de Lencastre.

View document
 
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Invasiveness of pneumococcal serotypes and clones circulating in Catalonia during heptavalente conjugate vaccine period View proposal
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