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   COLLABORATIVE PROJECTS

Interbio aims at promoting interdisciplinary and interregional research in the field of health & life sciences. Interbio can provide mobility support (transport & accommodation) for scientists interested in setting up collaborative research projects.
For more information, please contact your local WP3 coordinator.

WG1 Comparative proteome analysis of C. albicans strains SC5314 and CAI4

Partner A Member : Toulouse
Name of principal investigator : MONSARRAT
FirstName : Bernard
E-mail : Bernard.Monsarrat@ipbs.fr
Position : Staff
Affiliation Name : Institut de Pharmacologie et de Biologie Structurale
Road : 205 route de Narbonne
Postbox :
Town : Toulouse
Country : France
Partner B Member : Valencia
Name of principal investigator : DEL CASTILLO AGUDO
FirstName : Lucas
E-mail : lucas.castillo@uv.es
Position : Staff
Affiliation Name : Dep. Microbiología y Ecología; Fac. Farmacia; Universidad de Valencia
Road : Vicente Andres Estelles s/n
Postbox :
Town : Burjassot
Country : Espña
Request for financial support 1 scientist for 90 days (Partner B to Partner A)
Perspectives of extension of this common project to a more ambitious proposal to be submitted to funding agencies : Short term
Added value of the cooperation : I have establish contacts that can allow to further collaborative research projects in the area of fungal and Mycobacteria infections.

Summary of the proposal : Comparative proteome analysis of C. albicans strains SC5314 and CAI4

 

Proteomics is a rapidly evolving field. Initially devoted to protein identification, it is today aiming at an in-depth characterization of proteomes to reach functional proteomics. This leads to new challenges including the analysis of post-translational modifications playing a key role in protein function, the identification of protein complexes and the study of their dynamics, the quantitative determination of proteome variations upon various stimuli or in different cellular states, and the detection of low-abundance proteins of biological importance.

Over the past decade, mass spectrometry (MS) has represented a key technology for the analysis of biomolecules in general and of proteins in particular, becoming a central analytical tool for the development of proteomics.

The recent advances in MS instrumentation with the latest mass spectrometers such as the orbitrap offer unprecedented capabilities in terms of sensitivity, acquisition speed, and mass resolution, which open exciting new areas of proteomic applications. Combined with sophisticated biochemical methods for protein extraction, separation, and purification, and dedicated bioinformatics tools for data analysis, these MS-based strategies are becoming powerful complementary approaches to cellular, pharmacological, and other molecular techniques to contribute significantly to the understanding of complex regulatory protein networks.

In this context, the research projects of the group of Dr B. Monsarrat devoted to the analysis of biological systems involved mainly in cancer and inflammation trigger the development of state-of-the-art proteomic strategies. The principal aim of the Toulouse Proteomics Platform is to provide state-of-the-art techniques in proteomics to the scientific community.

Proteomics is a rapidly expanding field, where instrumentation in mass spectrometry and associated analytical and biochemical strategies are regularly improving.

The development of quantitative proteomic strategies have been so far successful thanks to improved mass spectrometers capabilities (high resolution, high acquisition speed, high sensitivity) and to new bioinformatic tools, and should develop further in the coming years. Toulouse Proteomics Platform is equipped with modern mass spectrometers and has increased its capacities in bioinformatics over the last 4 years.

 

Our group in Valencia work in yeast cell wall structure, in particular we are interested in the human yeast pathogens of the genus Candida and nearest species and in particular C. Albicans. Actualy more molecular studies in this species were carried out with the mutant strain CAI4, and his parental clinical strain SC5314. However Although CAI4 has been initially described as a auxotrophic mutant, abnormal phenotypes not associated to uracil auxotrophy have been soon observed.

Differences in the proteome of these strains can explain these differences and addimportant data for validation of molecular and clinic results carried out with this strain.

Protein samples of C. albicans strain CAI4 and his parental SC5314, had been used to contrastthe proteome of these strains in different conditions of culture, including iron depletion, oxidative and nutritional stress.

Comparison of proteome of these strains by complex mixture analysis allow us to identify ≈750 validate proteins by comparison of peptides sequences with C. albicans protein data bank at Pasteur institute. Comparison of proteins of cells grown in standard conditions, allows us to show important differences between both strains proteome, in particular a under expression of ribosomal proteins in The CAI4 strains.

Analysis of strips after SDS-PAGE separation and comparison of proteome in different stress conditions are still under bioinformatics analysis.

The contacts realised in Toulouse open the door for further collaborative projects with the diferents groups at the “Institut de Pharmacologie et de Biologie Structurale”

Antonio PINEDA-LUCENA, Valencia
Claudio GOMES, Lisboa
Using metabonomics to understand fatty acid oxidation disease View proposal
Miriam ROYO EXPOSITO, Barcelona
Ivan HUC, Bordeaux
Exploring delta-aromatic carboxamide foldamers and alpha-peptides compatibility View proposal
Salvador VENTURA ZAMORA, Barcelona
Ana VARELA COELHO, Lisboa
GT4.- Proteomic analysis of cellular responses to protein-aggregation-mediated stress View proposal
Olivier GADAL, Toulouse
Marc MARTI-RENOM, Valencia
Integrating probabilistic maps and 3C-based data for determining high-resolution 3D structure of yeast chromosomes View proposal
Yann ASTIER, Lisboa
Josep SAMITIER, Barcelona
Nanochannels for single molecule studies View proposal
Jacqueline GRIMA PETTENATI, Toulouse
Jorge PINTO PAIVA, Lisboa
The MicroRNAs in Eucalyptus tension wood world View proposal
Royo BEATRIZ , Lisboa
Gómez MONTSERRAT, Toulouse
Molybdenum complexes containing chiral oxazoline ligands: coordination and catalysis View proposal
Fernando ROCH, Toulouse
Elio SUCENA, Lisboa
Studying functional diversification and evolution of regulatory sequences after a gene duplication event View proposal
Gilles GUICHARD, Bordeaux
Fernando ALBERICIO, Barcelona
High-Throughput Screening of one-bead/one-compound (OBOC) foldamer libraries against protein targets View proposal
Bruno BRUNO VICTOR, Lisboa
Xavier XAVIER DAURA, Barcelona
Molecular Simulations of Hemagglutinin from Influenza virus View proposal
Bernard MONSARRAT, Toulouse
Lucas DEL CASTILLO AGUDO, Valencia
WG1 Comparative proteome analysis of C. albicans strains SC5314 and CAI4 View proposal
Peter FALLER, Toulouse
Claudio GOMES, Lisboa
Structural and functional aspects of the Cu/Fe-amyloid-beta complex View proposal
Egea GUSTAVO, Barcelona
Elisabeth GENOT, Bordeaux
TGFbeta signaling in cardiovascular alterations associated with the Marfan syndrome View proposal
Cecilia ARRAIANO, Lisboa
Muriel COCAIGN-BOUSQUET, Toulouse
Stabilome E coli View proposal
Muñoz-almagro CARMEN , Barcelona
Sa-leao RAQUEL, Lisboa
Invasiveness of pneumococcal serotypes and clones circulating in Catalonia during heptavalente conjugate vaccine period View proposal
Olga IRANZO, Lisboa
Rafel PROHENS, Barcelona
Study of the influence of peptide flexibility on metal ion coordination properties View proposal
Rita ABRANCHES, Lisboa
José maría SEGUÍ, Valencia
Investigating the subcellular trafficking of human recombinant proteins expressed in plants View proposal
Francesc PALAU, Valencia
Cláudio GOMES, Lisboa
Evaluation of mitochondrial beta-oxidation flavoenzymes in a cellular model of Friedreich ataxia View proposal
Jean-jacques TOULMÉ, Bordeaux
Claudio GOMES, Lisboa
Studies on human S100 protein-aptamer complexes View proposal
Justine BERTRAND-MICHEL, Toulouse
Gemma FABRIÀS, Barcelona
WP4 Sphingolipidomic technical exchange View proposal
Célia ROMÃO, Lisboa
Jordi juanhuix GIBERT, Barcelona
Introduction to the MX XALOC BL13 Beamline at ALBA View proposal
   

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