Interbio aims at promoting interdisciplinary and interregional research in the field of health & life sciences. Interbio can provide mobility support (transport & accommodation) for scientists interested in setting up collaborative research projects. For more information, please contact your local WP3 coordinator.
Nanochannels for single molecule studies
Perspectives of extension of this common project to a more ambitious proposal to be submitted to funding agencies : Short term Added value of the cooperation : This cooperation brings together the nanopore single molecule expertise of the Lisbon group with the lab on a chip microfluidic expertise of the Barcelona group, and the material science capacity of the Toulouse group
Summary of the proposal :
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We propose to build Nanochannels that consist in a much larger conduct than the nanopore, typically hundreds of nanometers to a micrometer in length. The aim is to monitor a single molecule for more than the few miliseconds a nanopore can afford. The length of the nanochannel would allow for electrode rings to be positioned at regular intervals along the channel path to detect the progress of a molecule down the nanochannel. Such ring electrodes can be plasmon resonance electrodes to measure field effects from the passage of a molecule. Ideally, we aim to couple electrophysiological and electrochemical measurements with direct single molecule fluorescence observations of fluorescently tagged molecules. The field driving the molecule down the nanochannel could also be alternated to have a single molecule move back and forth across the channel electrode. Therefore, instead of short single recordings of multiple molecules, one could achieve multiple recordings of a single molecule. We believe that this tool could deliver biophysical information about the molecule studied. Protein-protein interaction at the single molecule level could be revealed, protein DNA interactions, DNA fragments, RNA, small RNAs could also be studied.
As more applications are published on the use of nanopores for single molecule studies, it is important to create a technological platform allowing scientists from unrelated fields to aquire single nanopore analysis tools. This consortium proposes to further the understanding of nanopores and nanochannels, while creating a user friendly platform to pursue applications in medical diagnostic, DNA sequencing, and Bio-defence detection in collaboration with industry.